abstract：:Filamentous fungus Fusarium poae is a worldwide cause of the economically important disease Fusarium head blight of cereal grains. The fungus is itself commonly infected with a bisegmented dsRNA virus from the family Partitiviridae. For this study, we determined the structure of partitivirus Fusarium poae virus 1 (FpV...

journal_title：Journal of structural biology

authors： Tang J,Ochoa WF,Li H,Havens WM,Nibert ML,Ghabrial SA,Baker TS

更新日期：2010-12-01 00:00:00

abstract：:Producing purified human proteins with high yield and purity remains a considerable challenge. We describe the methods utilized in the Structural Genomics Consortium (SGC) in Oxford, resulting in successful purification of 48% of human proteins attempted; of those, the structures of approximately 40% were solved by X-...

journal_title：Journal of structural biology

authors： Savitsky P,Bray J,Cooper CD,Marsden BD,Mahajan P,Burgess-Brown NA,Gileadi O

更新日期：2010-10-01 00:00:00

abstract：:Raman spectroscopy was used to determine the mineralogical composition of the calcareous tubes of three species belonging to the family Cirratulidae. In all three cases, the tubes were found to be aragonitic, confirming previous inferences based on EDX and thin section studies, and corroborated by new EDX analyses rev...

journal_title：Journal of structural biology

authors： Taylor PD,Vinn O,Kudryavtsev A,Schopf JW

更新日期：2010-09-01 00:00:00

abstract：:Tobacco mosaic virus (TMV) is a plant virus with a highly ordered organisation and has been described in three different structural states: As stacked disks without RNA (X-ray crystallography), as a helical form with RNA (X-ray fibre diffraction) and as a second distinct helical form with RNA (cryo-EM). Here we presen...

journal_title：Journal of structural biology

authors： Clare DK,Orlova EV

更新日期：2010-09-01 00:00:00

abstract：:Fructokinase (FRK; EC 2.7.1.4) catalyzes the phosphorylation of d-fructose to d-fructose 6-phosphate (F6P). This irreversible and near rate-limiting step is a central and regulatory process in plants and bacteria, which channels fructose into a metabolically active state for glycolysis. Towards understanding the mecha...

journal_title：Journal of structural biology

authors： Chua TK,Seetharaman J,Kasprzak JM,Ng C,Patel BK,Love C,Bujnicki JM,Sivaraman J

更新日期：2010-09-01 00:00:00

abstract：:The microstructure and its crystallographic aspect of the shell of a limpet, Lottiakogamogai, have been investigated, as the first step to clarify the mechanism of shell formation in limpet. The shell consists of five distinct layers stacked along the shell thickness direction. Transmission electron microscopy (TEM) w...

journal_title：Journal of structural biology

authors： Suzuki M,Kameda J,Sasaki T,Saruwatari K,Nagasawa H,Kogure T

更新日期：2010-08-01 00:00:00

abstract：:Cryo-tomography in the electron microscope is unique in its ability to provide high-resolution, three-dimensional structural information about cells, organelles and macromolecules in a nearly native, frozen-hydrated state. However, the phase-contrast imaging method used in conventional cryo-electron tomography fails t...

journal_title：Journal of structural biology

authors： Danev R,Kanamaru S,Marko M,Nagayama K

更新日期：2010-08-01 00:00:00

abstract：:RCK is a cytoplasmic regulatory domain of calcium-gated potassium channels. Binding of Ca(2+) by RCK leads to channel activation through a series of yet unknown conformational changes. Structures of the K(+) channel, MthK, and its cytoplasmic RCK domain revealed two binding sites for Ca(2+) per dimer. We determined th...

journal_title：Journal of structural biology

authors： Dvir H,Valera E,Choe S

更新日期：2010-08-01 00:00:00

abstract：:Plasminogen activator inhibitor-1 (PAI-1) is a serine protease inhibitor (serpin) that plays an important role in cardiovascular disorders and tumor development. The potential role of PAI-1 as a drug target has been evaluated in various animal models (e.g. mouse and rat). Sensitivity to PAI-1 inhibitory agents varied ...

journal_title：Journal of structural biology

authors： Dewilde M,Van De Craen B,Compernolle G,Madsen JB,Strelkov S,Gils A,Declerck PJ

更新日期：2010-07-01 00:00:00

abstract：:Bdelloid rotifers are aquatic microinvertebrates able to cope with the loss of environmental water by entering dormancy, and are thus capable of living in temporary habitats. When water is evaporating, bdelloids contract into "tuns", silence metabolism and lose water from the body, a condition known as anhydrobiosis. ...

journal_title：Journal of structural biology

authors： Marotta R,Leasi F,Uggetti A,Ricci C,Melone G

更新日期：2010-07-01 00:00:00

abstract：:Electron tomography allows structural studies of cellular structures at molecular detail. Large 3D reconstructions are needed to meet the resolution requirements. The processing time to compute these large volumes may be considerable and so, high performance computing techniques have been used traditionally. This work...

journal_title：Journal of structural biology

authors： Agulleiro JI,Garzón EM,García I,Fernández JJ

更新日期：2010-06-01 00:00:00

abstract：:Folding and self-assembly of amyloid beta (Abeta) peptide are linked to Alzheimer's disease. To understand the initial stage of amyloid-beta peptide aggregation, conformational characteristics of monomers of wild-type (WT) Abeta(28-35) and its mutant peptides A30G and A30I were investigated using density functional th...

journal_title：Journal of structural biology

authors： Nagarajan S,Rajadas J,Malar EJ

更新日期：2010-06-01 00:00:00

abstract：:Corneal transparency is fundamental to the visual system, and is directly related to the ordered collagen fibril architecture that the cornea maintains. Proteoglycans, through their protein core and highly anionic glycosaminoglycan side chains, are thought to regulate the collagen organisation in the corneal stroma. T...

journal_title：Journal of structural biology

authors： Parfitt GJ,Pinali C,Young RD,Quantock AJ,Knupp C

更新日期：2010-05-01 00:00:00

abstract：:The rod domain of IF molecules has been characterized as four alpha-helical coiled-coil segments (1A, 1B, 2A and 2B), three linkers (L1, L12 and L2) and a stutter at the centre of segment 2B. Two of these breaks in coiled-coil continuity (L2 and stutter) have been modelled on the basis of structural data obtained from...

journal_title：Journal of structural biology

authors： Parry DA,Smith TA

更新日期：2010-05-01 00:00:00

abstract：:Four-helical bundles are the most abundant topological motif among helical folds. Their constituent helices show crossing angles that mainly cluster around +20 degrees (aligned) or -50 degrees (orthogonal). Bundles with all helices aligned are called 'square' and comprise four-helical coiled coils as their structurall...

journal_title：Journal of structural biology

authors： Dunin-Horkawicz S,Lupas AN

更新日期：2010-05-01 00:00:00

abstract：:Anti-TRAP (AT) protein regulates expression of tryptophan biosynthetic genes by binding to the trp RNA-binding attenuation protein (TRAP) and preventing its interaction with RNA. Bacillus subtilis AT forms trimers that can either interact with TRAP or can further assemble into dodecameric particles. To determine which...

journal_title：Journal of structural biology

authors： Shevtsov MB,Chen Y,Isupov MN,Leech A,Gollnick P,Antson AA

更新日期：2010-04-01 00:00:00

abstract：:Radiation damage is the primary factor that limits resolution in electron cryo-microscopy (cryo-EM) of frozen-hydrated biological samples. Negative effects of radiation damage are attenuated by cooling specimens to cryogenic temperatures using liquid nitrogen or liquid helium. We have examined the relationship between...

journal_title：Journal of structural biology

authors： Bammes BE,Jakana J,Schmid MF,Chiu W

更新日期：2010-03-01 00:00:00

abstract：:Amelogenin is believed to be involved in controlling the formation of the highly anisotropic and ordered hydroxyapatite crystallites that form enamel. The adsorption behavior of amelogenin proteins onto substrates is very important because protein-surface interactions are critical to its function. We have previously u...

journal_title：Journal of structural biology

authors： Tarasevich BJ,Lea S,Shaw WJ

更新日期：2010-03-01 00:00:00

abstract：:Lipocalin-type prostaglandin D synthase (L-PGDS) acts as both a PGD(2) synthase and an extracellular transporter for small lipophilic molecules. From a series of biochemical studies, it has been found that L-PGDS has an ability to bind a variety of lipophilic ligands such as biliverdin, bilirubin and retinoids in vitr...

journal_title：Journal of structural biology

authors： Miyamoto Y,Nishimura S,Inoue K,Shimamoto S,Yoshida T,Fukuhara A,Yamada M,Urade Y,Yagi N,Ohkubo T,Inui T

更新日期：2010-02-01 00:00:00

abstract：:To date, the calcareous tubes of serpulid marine worms have not been studied extensively in a biomineralization context. The structure and composition of the tube shell and adhesive cement of the marine tubeworm Hydroides dianthus were studied using a variety of characterization techniques, including powder XRD, FTIR,...

journal_title：Journal of structural biology

authors： Tanur AE,Gunari N,Sullan RM,Kavanagh CJ,Walker GC

更新日期：2010-02-01 00:00:00

abstract：:The recombinant dihydropyrimidinase from Sinorhizobium meliloti CECT4114 (SmelDhp) has been characterised and its crystal structure elucidated at 1.85A. The global architecture of the protein is reminiscent of that of the amidohydrolase superfamily, consisting of two domains; an (alpha/beta)(8) TIM-like barrel domain,...

journal_title：Journal of structural biology

authors： Martínez-Rodríguez S,Martínez-Gómez AI,Clemente-Jiménez JM,Rodríguez-Vico F,García-Ruíz JM,Las Heras-Vázquez FJ,Gavira JA

更新日期：2010-02-01 00:00:00

abstract：:The bacterial type II protein secretion (T2S) and type IV piliation (T4P) systems share several common features. In particular, it is well established that the T2S system requires the function of a pilus-like structure, called pseudopilus, which is built upon assembly of pilin-like subunits, called pseudopilins. Pilin...

journal_title：Journal of structural biology

authors： Alphonse S,Durand E,Douzi B,Waegele B,Darbon H,Filloux A,Voulhoux R,Bernard C

更新日期：2010-01-01 00:00:00

abstract：:The structural remodeling of collagen is important in biological processes such as fibrosis, developmental morphogenesis and wound repair. Highly ordered collagen macromolecules produce second harmonic generation signals without the need for any exogenous label. Conversely, the cellular components stained with exogeno...

journal_title：Journal of structural biology

authors： Abraham T,Carthy J,McManus B

更新日期：2010-01-01 00:00:00

abstract：:In many microorganisms, menaquinone is an essential lipid-soluble electron carrier. Recently, an alternative menaquinone biosynthetic pathway was found in some microorganisms [Hiratsuka, T., Furihata, K., Ishikawa, J., Yamashita, H., Itoh, N., Seto, H., Dairi, T., 2008. An alternative menaquinone biosynthetic pathway ...

journal_title：Journal of structural biology

authors： Arai R,Murayama K,Uchikubo-Kamo T,Nishimoto M,Toyama M,Kuramitsu S,Terada T,Shirouzu M,Yokoyama S

更新日期：2009-12-01 00:00:00

abstract：:The skeletons of demosponges, such as Ianthella basta, are known to be a composite material containing organic constituents. Here, we show that a filigree chitin-based scaffold is an integral component of the I. basta skeleton. These chitin-based scaffolds can be isolated from the sponge skeletons using an isolation a...

journal_title：Journal of structural biology

authors： Brunner E,Ehrlich H,Schupp P,Hedrich R,Hunoldt S,Kammer M,Machill S,Paasch S,Bazhenov VV,Kurek DV,Arnold T,Brockmann S,Ruhnow M,Born R

更新日期：2009-12-01 00:00:00

abstract：:Nanoparticle transport across cell membrane plays a crucial role in the development of drug delivery systems as well as in the toxicity response induced by nanoparticles. As hydrophilic nanoparticles interact with lipid membranes and are able to induce membrane perturbations, hypothetic mechanisms based on membrane cu...

journal_title：Journal of structural biology

authors： Le Bihan O,Bonnafous P,Marak L,Bickel T,Trépout S,Mornet S,De Haas F,Talbot H,Taveau JC,Lambert O

更新日期：2009-12-01 00:00:00

abstract：:Regulation of gene transcription in both prokaryotes and eukaryotes involves the formation of DNA-multiprotein complexes. These complexes build a precise three-dimensional topology allowing communication between distal regions of DNA. The switch from early to late transcription in bacteriophage Ø29 involves binding of...

journal_title：Journal of structural biology

authors： del Arroyo PG,Vélez M,Piétrement O,Salas M,Carrascosa JL,Camacho A

更新日期：2009-12-01 00:00:00

abstract：:In order to understand whole tooth behavior under load the biomechanical role of enamel and dentin has to be determined. We approach this question by comparing the deformation pattern and stiffness of intact teeth under load with the deformation pattern and stiffness of the same teeth after the enamel has been mechani...

journal_title：Journal of structural biology

authors： Barak MM,Geiger S,Chattah NL,Shahar R,Weiner S

更新日期：2009-12-01 00:00:00

abstract：:Chaperone-proteases are responsible for the processive breakdown of proteins in eukaryotic, archaeal and bacterial cells. They are composed of a cylinder-shaped protease lined on the interior with proteolytic sites and of ATPase rings that bind to the apical sides of the protease to control substrate entry. We present...

journal_title：Journal of structural biology

authors： Kolygo K,Ranjan N,Kress W,Striebel F,Hollenstein K,Neelsen K,Steiner M,Summer H,Weber-Ban E

更新日期：2009-11-01 00:00:00

abstract：:Citropin 1.1 is a basic, highly hydrophobic, 16-amino acid peptide (GLFDVIKKVASVIGGL-NH(2)), displaying wide-spectrum antimicrobial activities. In this paper we describe the synthesis and antimicrobial properties of citropin 1.1 and its 18 analogs constituting mostly truncated fragments of citropin 1.1. Moreover, we e...

journal_title：Journal of structural biology

authors： Sikorska E,Greber K,Rodziewicz-Motowidło S,Szultka L,Lukasiak J,Kamysz W

更新日期：2009-11-01 00:00:00

abstract：:TDP-43 is a RNA/DNA-binding protein structurally related to nuclear hnRNP proteins. Previous biochemical studies have shown that this nuclear protein plays a role in the regulation of gene transcription, alternative splicing and mRNA stability. Despite the ubiquitous distribution of TDP-43, the growing list of TDP-43 ...

journal_title：Journal of structural biology

authors： Casafont I,Bengoechea R,Tapia O,Berciano MT,Lafarga M

更新日期：2009-09-01 00:00:00

abstract：:We demonstrate in this work that scanning tunneling microscopy (STM) provides a useful approach to obtaining structural information about human islet amyloid polypeptide (hIAPP) and rat islet amyloid polypeptide (rIAPP) assembly on highly oriented pyrolytic graphite (HOPG) with sub-molecular resolution. The observed h...

journal_title：Journal of structural biology

authors： Mao X,Ma X,Liu L,Niu L,Yang Y,Wang C

更新日期：2009-09-01 00:00:00

abstract：:Virus particle 24 (VP24) is the smallest protein of the Ebola and Marburg virus genomes. Recent experiments show that Ebola VP24 blocks binding of tyrosine-phosphorylated STAT-1 homodimer (PY-STAT1) to the NPI-1 subfamily of importin alpha, thereby preventing nuclear accumulation of this interferon-promoting transcrip...

journal_title：Journal of structural biology

authors： Lee MS,Lebeda FJ,Olson MA

更新日期：2009-08-01 00:00:00

abstract：:Biophysical cues such as substrate modulus have been shown to influence a variety of cell behaviors. We have determined the elastic modulus of the anterior basement membrane and Descemet's membrane of the human cornea with atomic force microscopy (AFM). A spherical probe was used with a radius approximating that of a ...

journal_title：Journal of structural biology

authors： Last JA,Liliensiek SJ,Nealey PF,Murphy CJ

更新日期：2009-07-01 00:00:00

abstract：:Ion-abrasion scanning electron microscopy (IASEM) takes advantage of focused ion beams to abrade thin sections from the surface of bulk specimens, coupled with SEM to image the surface of each section, enabling 3D reconstructions of subcellular architecture at approximately 30nm resolution. Here, we report the first a...

journal_title：Journal of structural biology

authors： Hildebrand M,Kim S,Shi D,Scott K,Subramaniam S

更新日期：2009-06-01 00:00:00

abstract：:Commonly employed data models for maximum likelihood refinement of electron microscopy images behave poorly in the presence of normalization errors. Small variations in background mean or signal brightness are relatively common in cryo-electron microscopy data, and varying signal-to-noise ratios or artifacts in the im...

journal_title：Journal of structural biology

authors： Scheres SH,Valle M,Grob P,Nogales E,Carazo JM

更新日期：2009-05-01 00:00:00

abstract：:In the cornea, the precise organisation of fibrillar collagen and associated proteoglycans comprising the stromal extracellular matrix plays a major role in governing tissue form and function. Recently, abnormal collagen alignment was noted in the misshapen corneas of mature chickens affected by the retinopathy, globe...

journal_title：Journal of structural biology

authors： Boote C,Hayes S,Young RD,Kamma-Lorger CS,Hocking PM,Elsheikh A,Inglehearn CF,Ali M,Meek KM

更新日期：2009-05-01 00:00:00

abstract：:Ferritin, the major iron storage protein, has dual functions; it sequesters redox activity of intracellular iron and facilitates iron turn-over. Here we present high angle annular dark field (HAADF) images from individual hepatic ferritin cores within tissue sections, these images were obtained using spherical aberrat...

journal_title：Journal of structural biology

authors： Pan YH,Sader K,Powell JJ,Bleloch A,Gass M,Trinick J,Warley A,Li A,Brydson R,Brown A

更新日期：2009-04-01 00:00:00

abstract：:Three-dimensional electron microscopy allows direct visualization of biological macromolecules close to their native state. The high impact of this technique in the structural biology field is highly correlated with the development of new image processing algorithms. In order to achieve subnanometer resolution, the si...

journal_title：Journal of structural biology

authors： Bilbao-Castro JR,Marabini R,Sorzano CO,García I,Carazo JM,Fernández JJ

更新日期：2009-01-01 00:00:00

abstract：:The hypothesis that distinct chromatin domains expand and are remodelled differently when they undergo transcription, replication or cell cycle processes is well accepted. The condensation changes by which chromosomes are transformed at the metaphase-interphase transition are especially interesting and therefore exten...

journal_title：Journal of structural biology

authors： Batram C,Baddeley D,Kreth G,Cremer C

更新日期：2008-12-01 00:00:00